《植物生理学报》 2012, 48(7): 683-688

高温胁迫下2,4-表油菜素内酯对甜瓜幼苗生理及光合特性的影响

张永平, 陈幼源*, 杨少军

上海市农业科学院园艺研究所, 上海市设施园艺技术重点实验室, 上海201403

通信作者：陈幼源；E-mail: yy12@saas.sh.cn；Tel: 021-52630133

摘　要：

研究了高温胁迫下不同浓度2,4-表油菜素内酯(EBR)处理对甜瓜幼苗生长、叶片抗氧化酶活性和光合作用的影响。 结果显示0.5~1.5 mg•L-1 EBR处理能有效缓解甜瓜幼苗高温胁迫下受到的伤害, 显著促进了幼苗生长, 提高了叶片抗氧化酶 活性、脯氨酸和可溶性蛋白含量、净光合速率(Pn)和气孔导度(Gs); 降低了丙二醛(MDA)含量, 胞间CO2浓度(Ci)和蒸腾速率(Tr)。研究结果表明, EBR缓解甜瓜幼苗高温胁迫具有剂量效应, 以1.0mg•L-1EBR的效果较好, 有利于甜瓜幼苗在高温胁迫下抗氧化酶活性的维持和对光能的捕获与转换, 促进生长。

关键词：2,4-表油菜素内酯; 甜瓜; 高温胁迫; 生理特性; 光合作用

收稿：2012-03-12   修定：2012-06-01

资助：上海市科技兴农重点公关项目[沪农科攻字(2009)第2-1号]、上海市闵行区科技项目(2011MH001)和上海市西甜瓜产业体系项目。

Effects of 2,4-Epibrassinolide on Physiological Characteristics and Photosynthesis of Melon Seedlings under High Temperature Stress

ZHANG Yong-Ping, CHEN You-Yuan*, YANG Shao-Jun

Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China

Corresponding author: CHEN You-Yuan; E-mail: yy12@saas.sh.cn; Tel: 021-52630133

Abstract:

Under high temperature condition, the effects of 2,4-epibrassinolide (EBR) on the growth, activities of antioxidant enzymes and photosynthesis in melon seedling leaves were investigated. The results showed that the treatment of 0.5–1.5 mg·L^-1 EBR could alleviate high temperature damage effectively. At these concentrations, the seedling growth, activities of antioxidant enzymes, contents of proline and soluble protein, net photosynthetic rate (Pn) and stomatal conductance (Gs) in leaves were significantly increased, while malondialdehyde (MDA) content, intercellular carbon dioxide concentration (Ci) and transpiration rate (Tr) in leaves were decreased. The above results indicated that there were dosage effect of EBR on the alleviation of high temperature in melon seedlings, and the better alleviating effect on high temperature damage was 1.0 mg·L^-1 EBR. EBR was favorable for the seedlings to increase the growth, maintain antioxidant enzyme activity, capture and converse solar energy, and thus improving melon growth.

Key words: 2,4-epibrassinolide; melon; high temperature stress; physiological characteristics; photosynthesis